gin queen is a queen bee that has not mated with a drone. Virgins are intermediate in size between workers and mated, laying queens, and are much more active than the latter. They are hard to spot while inspecting a frame, because they run across the comb, climbing over worker bees if necessary, and may even take flight if sufficiently disturbed. Virgin queens can often be found clinging to the walls or corners of a hive during inspections. Virgin queens appear to have little queen pheromone and often do not appear to be recognized as queens by the workers. A virgin queen in her first few hours after emergence can be placed into the entrance of any queenless hive or nuc and acceptance is usually very good, whereas a mated queen is usually recognized as a stranger and runs a high risk of being killed by the older workers. When a young virgin queen emerges from a queen cell, she will generally seek out virgin queen rivals and attempt to kill them . Virgin queens will quickly find and kill (by stinging) any other emerged virgin queen (or be dispatched themselves), as well as any unemerged queens. Queen cells that are opened on the side indicate that a virgin queen was likely killed by a rival virgin queen. When a colony remains in swarm mode afme swarm has left, the workers may prevent virgins from fighting and one or several virgins may go with after-swarms. Other virgins may stay behind with the remnant of the hive. Some virgins have been seen to escape the hive to avoid being killed and seek out another without a queen, such as in the eusocial bee Melipona scutellaris. This can contain multiple virgin queens. When the after-swarm settles into a new home, the virgins will then resume normal behavior and fight to the death until only one remains. If the prime swar